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A batch enzyme reaction system utilising the industrially important glucose isomerisation reaction (converting glucose to fructose) catalysed by glucose isomerase. The purpose of the unit is to demonstrate batch enzyme kinetics and enzyme characteristics. The reaction takes place inside a stirred vessel where the stirrer itself is a porous basket inside which the enzyme is immobilised. A polarimeter device which is integral to the unit monitors the glucose and fructose concentrations with time. Teaching exercises are included to familiarise the students with the following topics: > Determination of Michaelis-Menten constants and specific enzyme activity through Michaelis-Menten and Lineweaver-Burke plots |
Chromatography is a very widely used separation process in the chemical and biochemical engineering fields. It is a highly selective process capable of separating components of closely similar physical and chemical properties. It is important both at processing and process analysis scales. The system demonstrates an industrially important type of chromatography, namely low pressure liquid chromatography of which there are several varieties including ion-exchange, size-exclusion, hydrophobic interaction and affinity chromatography. Each of these types uses a different method of separation and respectively these are: particle charge, particle size and shape, molecule hydrophobicity, and biological specificity. The system includes detailed experimental procedures for size exclusion chromatography particularly in relation to the separation of proteins and dyes. However, it may be used to investigate other types of low pressure liquid chromatography. Size exclusion chromatography uses porous beads to separate particles of different sizes. The size range of the pores in the beads defines whether particles are totally excluded, partially excluded or whether they have full entry into all beads. Particles that are totally excluded have the shortest residence time in the column whereas particles that have full entry into the beads have the longest residence time. Partially excluded particles have residence times between the two. On this basis particles of different sizes are separated.A bench top unit comprising a vacuum formed ABS plastic plinth with integral electrical console. > Two borosilicate glass chromatography columns (250mm long and 10mm internal diameter) fitted with septum injectors and fed by a 3-channel peristaltic pump. > Syringe and needles for sample injection. > Fraction collector. > Analysis using a UV optical flow cell. > Protection devices for all electrical circuits. > Two displays: Optical absorbance, Timer display for fraction collector. > Sensor signals are routed to the USB port for connection to a PC. > Comprehensive instruction manual with detailed procedures and laboratory teaching exercises. |
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Mr. Varun Chopra
(CEO)
Edutek House, 29- B, Inderpuri, Ambala Cantt
Ambala,
Haryana -
133 001,
India
Telephone: +(91)-(171)-2654329
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